rabbit anti atp1b2  (Alomone Labs)

Journal: BMC Cancer

Article Title: Glioma malignancy is linked to interdependent and inverse AMOG and L1 adhesion molecule expression

doi: 10.1186/s12885-019-6091-5

Figure Lengend Snippet: Antibodies used for Western blot analysis

Article Snippet: Sections were incubated with rabbit polyclonal anti-ATP1B2/AMOG antibody (Thermo Scientific, catalog no. PA5–26279, 1:50, Rockford, USA) overnight at 4 °C PBS and incubated with biotinylated secondary antibody, and streptavidin-peroxidase conjugate according to the manufacturer’s instructions (Zhong Shan Golden Bridge Biotechnology, catalog no. PV-9000-D, Beijing, China).

Techniques: Western Blot

Journal: BMC Cancer

Article Title: Glioma malignancy is linked to interdependent and inverse AMOG and L1 adhesion molecule expression

doi: 10.1186/s12885-019-6091-5

Figure Lengend Snippet: Antibodies used for Western blot analysis

Article Snippet: After an overnight incubation, the cells were treated with L1 siRNA at 0, 5, 10 or 20 nM for 48 h. Cells were then fixed with 4% formaldehyde in PBS for 15 min, blocked with normal donkey serum and incubated overnight at 4 °C with monoclonal mouse anti-human L1 antibody (R&D Systems, MAB777, 1:200) or polyclonal rabbit anti-human ATP1B2/AMOG antibody (Thermo Scientific, PA5–26279, 1:200).

Techniques: Western Blot

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells

doi: 10.1016/j.jcmgh.2017.02.011

Figure Lengend Snippet: Growth of proliferative 2D monolayer of murine epithelium on surface of collagen hydrogel. ( A ) Workflow for subculturing 2-D monolayers. Monolayers are dissociated from collagen and split. Brightfield images ( bottom ) show cell preparation at different workflow stages. ( B ) Time lapse fluorescence (DsRed) images of crypt-derived cells on collagen at passage number 0 (P0, crypts) and passage number 5 (P5). Scale bar =100 μm. ( C ) Karyotype analysis of 2-D monolayer at passage number 5 showing a normal karyotype. ( D ) SEM image of monolayer on collagen gel at day 3 of culture. ( E ) Fluorescence image of cross section through the monolayer immunostained for actin ( green ) and β-catenin ( red ). Distribution of β-catenin (an intracellular protein) demonstrated columnar-shaped cells with height of 9.4 ± 0.8 μm and width of 7.5 ± 0.9 μm (n = 10). ( F ) High magnification view of subregion of ( D ). ( G ) High magnification view of subregion of ( E ). ( H ) Staining for ZO-1, E-cadherin, or occludin (red) ; villin or actin (green) ; integrin-β4 and NA + /K + -ATPase (red) . Nuclei (blue) .

Article Snippet: Primary antibodies were rabbit α-Muc2 (1:200, Santa Cruz sc-15334; Santa Cruz Technology, Santa Cruz, CA), rabbit α-ChgA (1:1000, Abcam ab15160; Cambridge, UK), rabbit α-Sox9 (1:100, Millipore AB5535; Billerica, MA), rabbit α-villin (1:200, Santa Cruz sc-28283), rabbit α-ZO-1 (1:100, Proteintech 21773-1-AP; Wuhan, China), rabbit α-E-cadherin (1:100, Proteintech 20874-1-AP), rabbit α-occludin (1:100, Proteintech 13409-1-AP), rabbit α-NA + /K + -ATPase (1:100, Proteintech 22338-1-AP), rabbit α-integrin-β4 (1:200, Santa Cruz sc-9090), and rabbit α-β-catenin (1:200, Santa Cruz sc-7199).

Techniques: Subculturing Assay, Fluorescence, Derivative Assay, Staining